# ABI Core Facilities & Usage Fees

Overview of ABI research core facilities, equipment, and instrumentation.

# Core Facilities

# Overview of Equipment

- [ABI Core Units Equipment List (July 2025)](https://kb.astate.edu/attachments/1303)

# Animal Cell Culture

BSC: Biological Safety Cabinet

CBH: Clean Bench Hood

- ##### 1.1- BSC, NuAire, Class II, type A2, NU-425-600 (ABI 237)
- ##### 1.2- BSC, NuAire, Class II, NU-437-600 (ABI 246)
- ##### 1.3- BSC, NuAire, Class II, type A2, N4-437-600 (ABI 251)
- ##### 1.4- BSC, The Baker Company, SterilGARD® III Advance0, SG603, modified from Class III to Class II (ABI 256)
- ##### 1.5- CBH, Labconco, model 3610004 (ABI 256)
- ##### 1.6- BSC, Labconco, Class II, 3440809 (ABI 266)
- ##### 1.7- BSC, Thermo Electron Corporation, Class II, Type A2, 1284 (ABI 346)
- ##### 1.8- CBH, The Baker Company, EdgeGARD, EG-6320, 6 ft (ABI 346)
- ##### 1.9- BSC, Labconco, Logic, model 3460009 (ABI 356)
- ##### 1.10- CBH, Thermo, Heraguard ECO Clean (ABI 356)
- ##### 1.11- CO2 incubator, Thermo Fisher Scientific, HERAcell Vios 160i (9 units: ABI 2x246, 2x251, 2x256, 1x346, 2x356)
- ##### 1.12- CO2 incubator, VWR, Symphony, Cat # 98000-362 (ABI 266)
- ##### 1.13- CO2 incubator, NuAire, NU-5510, single chamber (ABI 356)
- ##### 1.14- Table-top centrifuge, Beckman, Avanti J-15R (4 units: ABI 246, 256, 346, 356)
- ##### 1.15- Table-top centrifuge, Beckman, Allegra™ X-22R (ABI 251)
- ##### 1.16- Table-top centrifuge, Thermo Scientific, CL2 (ABI 266)
- ##### 1.17- Automated cell counter, Invitrogen, Countess™, C10281 (ABI 356)
- ##### 1.18- Automated cell counter, Invitrogen (Life Tech) system, Countess™ II FL (3 units: ABI 251, 256, 356)
- ##### 1.19- Inverted Microscope, Nikon, Eclipse TS 100 (ABI 246)
- ##### 1.20- Inverted Microscope, Zeiss, Primo Vert w/Zeiss camera ERc 5s (ABI 251, 256, 356)
- ##### 1.21- Inverted Microscope, Zeiss, Primo Vert 3841000390 (ABI 266)
- ##### 1.22- Water bath incubator, FisherBrand, Isotemp GPD 20 (ABI 246)
- ##### 1.23- Water bath incubator, Fisher Scientific, Isotemp 110, Cat # 15-460-10 (ABI 251)
- ##### 1.24- Water bath incubator, Fisher Scientific, Isotemp SWB15 (ABI 251)
- ##### 1.25- Water bath incubator, Fisher Scientific, Isotemp 110 (ABI 256)
- ##### 1.26- Water bath incubator, Thermo Electron Corp., Precision, Series 280, model 2841 (ABI 266)
- ##### 1.27- Water bath incubator, Thermo Electron Corp., Precision, Series 280 (ABI 246) 1.28. Water bath incubator, FisherBrand, Isotemp GPD 15D (ABI 356)
- ##### 1.29- Refrigerator (mini) (+4 °C), General Electric, black (ABI 246)
- ##### 1.30- Refrigerator/Freezer (+4 °C/-20 °C), Fisher Scientific, Isotemp,13-986-106A (ABI 251)
- ##### 1.31- Refrigerator (mini) (+4 °C), Fisher Scientific, 05LREEFSA, white (ABI 251)
- ##### 1.32- Refrigerator, chromatographic (+4 °C), Fisher Scientific, 13-986-138G (ABI 256)
- ##### 1.33- Freezer (-20 °C), Thermo Scientific, 3767 A (ABI 256)
- ##### 1.34- Refrigerator (+4 °C/-20 °C), FisherBrand, Isotemp, 13-9940291, model 18LCEEFSA (ABI 266)
- ##### 1.35- Refrigerator (+4 °C/-20 °C), Fisher Scientific, 13-986-106A (ABI 356)
- ##### 1.36- Refrigerator (+4 °C/-20 °C), FisherBrand, GT18LCEEF8A (ABI 356) ABI will provide gas CO2, annual certification for the cabinets and hoods, bleach, and ethanol.

# Flow Cytometry

- ##### 2.1- Flow cytometer/Cell sorter, BD Biosciences, FACSAria™
- ##### 2.2- Computer workstation with BD FACSDiva™ software
- ##### 2.3- Spare computer workstation with FCS Express™ 7 Professional software (De Novo Software)

This instrument is installed in ABI 244. ABI will provide sheath fluid, ethanol, bleach, calibration standards, plastic sample tubes, nozzles, O-rings, and groove chambers.

# Histology

- ##### 3.1- Cryostat, Epredia, HM525 NX

[![histology-cryostat2.jpg](https://kb.astate.edu/uploads/images/gallery/2025-12/scaled-1680-/histology-cryostat2.jpg)](https://kb.astate.edu/uploads/images/gallery/2025-12/histology-cryostat2.jpg)

- ##### 3.2-Automated rotary microtome, Leica, HistoCore AUTOCUT

[![histology-microtome.jpg](https://kb.astate.edu/uploads/images/gallery/2025-12/scaled-1680-/histology-microtome.jpg)](https://kb.astate.edu/uploads/images/gallery/2025-12/histology-microtome.jpg)

- ##### 3.3-Semi-enclosed benchtop tissue processor, Leica, TP1020

All instruments are installed in ABI 244.

# Imaging

ABI will provide lens paper, ethanol, immersion oil, gas CO2, and liquid CO2.

- ##### 4.1- Scanning Electron Microscope (SEM), sec SNE-4500M Plus (ABI 250)
    
    
    - Attachments:  
        Critical Point Dryer, Autosamdri-815, Series A  
        Energy Dispersive X-Ray Spectroscope, Bruker, SCU, Scan Generator (EDS, EDX, EDXS, XEDS)  
        SOTER™ condenser  
        Vacuum pump  
        Air compressor  
        Antivibration table  
        Computer workstation w/NanoEye and ESPRIT Compact software
- ##### 4.2- Bioimager, Agilent/BioTek, Cytation 5 (ABI 247)
    
    
    - Attachments:  
        Controller for CO2/O2 gas  
        Computer workstation with Gen5 software  
        Spare computer workstation for data analyses
- ##### 4.3- Upright microscope, Nikon, Eclipse E800 (ABI 247)
    
    
    - Attachments:  
        Roper Scientifics Camera, Photometrics, CoolSnap HQ  
        Computer workstation w/Nikon Elements software
- ##### 4.4- Upright microscope, Leica, DMR-type:020-525.732 (ABI 250)
    
    
    - Attachment:  
        Digital camera, Leica, model DFC340 FX
- ##### 4.5- Upright microscope w/infrared detection (Smiths Detection), Olympus, Illuminat IR II FL (ABI 247)
    
    
    - Attachments:  
        Smith's Detection adapter (Infrared), ILLUMINAT IR II FL, BX51TRF  
        Color View Camera
- ##### 4.6- Upright microscope, Nikon, Eclipse E400 (ABI 250)
- ##### 4.7- Upright microscope, Zeiss, Axioskop (ABI 247)
- ##### 4.8- Upright microscope, Fisher Scientific, Micromaster (ABI 250)
- ##### 4.9- Inverted microscope, Zeiss, AXIO Vert.A1 (ABI 247)
    
    
    - Attachments:  
        Fluorescence filters: DAPI, Hoescht Alexa Fluor 350 (375/460), EGFP, FITC, Cy2, Alexa Fluor 488 (480/535), TRITC, Cy3, TagRFP, Alexa Fluor 546 (540/605)  
        Microscope camera, Zeiss, Axiocam 305 mono  
        Computer workstation with Zeiss, ZEN software
- ##### 4.10- Inverted microscope, Nikon, Eclipse TE2000u (T-DH) (ABI 247)
    
    
    - Attachments:  
        Nikon Digital DS-Qi1Mc camera (DS cooled camera head)  
        Nikon Digital DS-Fi2 color camera  
         Led light source X-Cite 120 LED (Lumen Dynamics Group Inc., Model XT120L)  
        Computer workstation w/Nikon Elements software
- ##### 4.11- Inverted microscope, Zeiss, Axiovert 40C (ABI 250)
- ##### 4.12- Inverted microscope, Amscope, IN300TC (ABI 250)
- ##### 4.13- Dissection microscope, Zeiss, Stemi 2000-C (ABI 344)
    
    
    - Attachments:  
        Digital camera, Zeiss, ERc 5s  
        Cold light source, Zeiss, KL 1500 LCD (two flexible light guides)
- ##### 4.14. Dissection microscope, Nikon, SMZ 1500 (P-BERG) (ABI 250)
    
    
    - Attachments:  
        Color digital camera, Scion Corporation D100, model CFW-1312C  
        Single arm boom stand (heavy base)
- ##### 4.15. Dissection microscope, Leica, MZ6 (ABI 250)

# Mass Spectroscopy

- ##### 5.1- HPLC/Mass Spectrometer, Thermo/Dionex, Ultimate 3000/LTQ XL
    
    
    - Components:  
        Pump, LPG-34000RS
        
        Autosampler, WPS-3000TRS
        
        Column oven, TCC-3000RS
        
        UV-visible detector, DAD-3000
        
        Mass Spectrometer, linear ion trap, LTQ-XL
        
        Nitrogen generator, Peak Scientific, NM 32LA

This system is installed in ABI 333. ABI will provide helium, a nitrogen generator, calibration standards, and a vacuum system.

# Molecular Biology

- ##### 6.1- Purifier Vertical Clean Bench, Fisher Scientific, FB-PCR2 (ABI 244)
- ##### 6.2- Purifier Vertical Clean Bench Labconco, Cat # 3970301
- ##### 6.3- DNA/RNA/protein quantifier, Thermo, NanoDrop One<sup>©</sup>
- ##### 6.4- DNA/RNA/protein quantifier, Thermo Fisher Scientific, Nanodrop ND-8000
- ##### 6.5- Thermal cycler, Applied Biosystems, Veriti 96 well
- ##### 6.6- Real-time PCR system, Bio-Rad, CFX Opus 96 (96-well plates)
- ##### 6.7- Real-time PCR system, Bio-Rad, C1000 + CFX96 (96-well plates)
- ##### 6.8- Real-time PCR system, Bio-Rad, C1000 + CFX384 (384-well plates)
- ##### 6.9- Automated liquid handler, Eppendorf, epMotion 5075 (ABI 354)
- ##### 6.10- Automated electrophoresis system, Agilent, TapeStation 4150
- ##### 6.11. Automated electrophoresis system, Agilent, BioAnalyzer 2100

Except for items 6.1 and 6.9, all instruments are installed in ABI 344.

# Plant Cell and Tissue Culture

- ##### 7.1- Clean bench hood, The Baker Company, EdgeGARD - EG-8252, 8 ft (ABI 344)
- ##### 7.2- Clean bench hood, The Baker Company, EdgeGARD - EG-6252 (ABI 351)
- ##### 7.3- Clean bench hood, The Baker Company, EdgeGARD - EG-63201 (ABI 351)
- ##### 7.4- Clean bench hood, NuAire, Series 14 - NU-301-630 (ABI 366)
- ##### 7.5- Clean bench hood, NuAire, Series 20 - NU-301-630 (ABI 366)
- ##### 7.6- Cell transformation system assisted by helium (Biolistic® Particle Delivery System), Bio-Rad, PDS-1000/He (ABI 346)

ABI will provide helium and annual certification for the hood.

# Radioactivity

- ##### 8.1- Scintillation counter, Beckman Coulter, LS 6500
- ##### 8.2- Geiger-Müller counter, Ludlum Measurements, Inc., Model 3
- ##### 8.3- Table-top centrifuge, Beckman, Avanti J-15R
- ##### 8.4- Hybridization oven, single chamber, Techne, FHB2DP

The Radioactivity Room is located at ABI 359. ABI will provide the annual recertification of the scintillation counter.

# General Support

- ##### 9.1- Ultracentrifuge, Beckman Coulter, Optima L-90K (ABI 338)
- ##### 9.2- High-speed centrifuge, floor-type, Sorvall Evolution 208-60 LCD (ABI 238)
- ##### 9.3- High-speed centrifuge, floor-type, Sorvall, RC 5C Plus (ABI 258)
- ##### 9.4- High-speed centrifuge, floor-type, Sorvall, RC 5C Plus (ABI 338)
- ##### 9.5- High-speed centrifuge, floor-type, Thermo, Lynx 6000, cat # 75006590 (ABI 358)
- ##### 9.6- Microplate reader UV-vis spectrum, BioTek, Epoch (ABI 244, 344)
- ##### 9.7- Image analyzer, Li-Cor, Odyssey Fc (ABI 331)
- ##### 9.8- Automatic film developer, Konica Minolta, SRX-101A (ABI 331)
- ##### 9.9- Microplate reader, UV-visible-luminescence-fluorescence, Agilent/BioTek, Synergy HTX. Fluorescence filters: 360/460, 485/528, and 560/600 nm (ABI 247)
- ##### 9.10- Microanalytical scale, Mettler Toledo, XR6TUD5 (ABI 337)
- ##### 9.11- Freeze dryer system (lyophilizer), Labconco, FreeZone 4.5L (-105 °C) (ABI 338)
- ##### 9.12- Vacuum concentrator, Thermo/SpeedVac SPD 120 (ABI 333)
    
    
    - Attachments:

Refrigerated VaporTrap, Savant RVT5105

Vacuum pump

- ##### 9.13. Vacuum concentrator, Thermo Electron Corporation SPD 131 DDA (ABI 338)
    
    
    - Attachments:

Universal Vacuum System Plus, Savant UVS800DDA

# Liquid Nitrogen

10.- The outlet for liquid nitrogen is in the ABI loading area, next to the entrance to the Animal Facility, ABI A100.

Laboratories are responsible for dewars and cryotanks.

# Small Animal Facility

- ##### 11.1- Cage and rack washer, BeterBuilt®, R620L Series (A103)
- ##### 11.2- Gravity steam sterilizer (autoclave), GETINGE, 522LS (A103)
- ##### 11.3- Water purification system, ELGA, Purelab Classic, 18.2 mOhm.cm (A103)
- ##### 11.4- Anesthesia machine, VETEQUIP, Impac6 (2 units in A110, A113)
- ##### 11.5- Ventilated racks for cages, 40/80 (2 units in A114)
- ##### 11.6- Ventilated racks for cages, 64/128 (3 units in A114)
- ##### 11.7- Metal rack for animal cages, Lab Products, Inc. Supermouse 1800, Allerzone, 60/120 Cage (A117)

# Greenhouses

- ##### 12.1- Four greenhouses equipped with a computer-assisted system for controlling photoperiod, humidity, and temperature (ABI 411, 411B, 411C, 411D)
- ##### 12.2- Cold room for seed storage (ABI 404)
- ##### 12.3- Room for soil and plant transfer (ABI 409)
- ##### 12.4- Autoclave for plant materials/soil, GETINGE, Model 733LS (ABI 411A)
- ##### 12.5- Water purification system by reverse osmosis, Watts, HS-1000PT-SKH36MNA445AX (ABI 411A)

# Plant Growth Chambers

- ##### 13.1- Plant growth incubator, Conviron, PGC20, twin chambers, 2 levels (4 units)
- ##### 13.2- Plant growth incubator, Conviron, E7/2, top/bottom chambers (1 unit)
- ##### 13.3- Plant growth incubator, Conviron, ATC40, twin chambers, 2 levels (2 units)
- ##### 13.4- Plant growth incubator, Conviron, ATC60, twin chambers, 3 levels (4 units)
- ##### 13.5- Plant growth incubator, Conviron, TC80, twin chambers, 4 levels (1 unit)
- ##### 13.6- Plant growth incubator, Conviron, Adaptis A1000 (1 unit)
- ##### 13.7- Plant growth incubator, Percival, AR41L3 (2 units)
- ##### 13.8- Walk-in plant growth chambers, Percival, DS-3678-CSN (2 units)

All equipment is installed in ABI 140. All plant growth chambers are connected to a computer-assisted system for controlling temperature, humidity, and photoperiod.

# Infiltration Room

- ##### 14.1- Plant growth incubator, Conviron, Adaptis A1000 (2 units in ABI 141A)

# Other

- ##### 15.1- Water purification system by reverse osmosis, USFilter, VROLC01BX (1 unit in ABI 138)
- ##### 15.2- Water disinfection system, Ultraviolet, Aquafine® (2 units in ABI 138)
- ##### 15.3- Autoclave, STERIS®, Amsco® Century™, SV-120 (3 units in ABI 142)
- ##### 15.4- Laboratory glassware washer, STERIS®, Reliance® 500 (2 units in ABI 142)
- ##### 15.5- Water purification system, ELGA, Purelab Classic, 18.2 mOhm.cm (8 units across the labs: ABI 234, 244, 253, 261, 335, 344, 353, 361)
- ##### 15.6- Ice machines (3 units in ABI 142, 259, 358)
- ##### 15.7- Backup refrigerators at +4 °C, chromatographic type (5 units in ABI 3x238, 258, 358)
- ##### 15.8- Backup refrigerator at +4 °C, white (1 unit in ABI 238)
- ##### 15.9- Backup freezers at -20 °C (3 units in ABI 2x258, 358)
- ##### 15.10- Backup freezers at -80 °C (6 units in ABI 238, 2x258, 338, 2x358)

# ABI Usage Fees

Here you'll find fees for ABI Core facilities use, maintenance, and availability.

# Summary

- [ABI User Fees Summary - July 2025 (PDF)](https://kb.astate.edu/attachments/609)