# Contents

Chapter 1 Guidance, Regulations and Roles of Key Personnel   
1.1 Introduction  
1.2 Guidance  
1.3 Regulations  
1.4 Roles of Key Personnel  
1.4.1 Institutional Biosafety Committee (IBC)  
1.4.2 Biological Safety Officer  
1.4.3 Principal Investigator   
Chapter 2 Biological Risk Assessment  
2.1 Introduction  
2.2 Hazardous Characteristics of Agents   
2.2.1 Principal Hazardous Characteristics  
2.2.2 Risk Groups   
2.2.3 Resources   
2.3 Risk Assessment for Human Cell Lines and Human-Derived Materials   
2.4 Risk Assessment for Other Agents   
Chapter 3- Principles of Biosafety   
3.1 Introduction  
3.2 Engineering Controls   
3.3 Personal Protective Equipment   
3.4 Facility Construction and Design   
Chapter 4- Laboratory Biosafety Level Criteria   
4.1 Introduction   
4.2 Biosafety Level 1   
4.3 Biosafety Level 2   
4.4 Biosafety Level 3   
4.5 Biosafety Level 4   
4.6 Multi-User Research Labs   
Chapter 5- Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities  
Chapter 6- Select Agents/ Toxins and Principles of Laboratory Biosecurity   
Chapter 7- Medical Surveillance   
Chapter 8 Emergencies and Reporting   
8.1 Spills   
8.1.1 General   
8.1.2 Spills outside of a containment device   
8.1.3 Spills inside of a Biological Safety Cabinet (BSC)   
8.1.4 Spills in a centrifuge   
8.1.5 Biological/Radioactive emergencies/spills  
8.2 Exposure to Biohazardous Agents or Material   
8.3 Security incidents   
Chapter 9 Biohazardous Waste Management  
9.1 Scope   
9.2 Biohazardous Waste Definition   
9.3 Responsibilities  
9.3.1 Principal Investigator or Faculty in Charge of Lab  
9.3.2 Biological Safety Officer (BSO)   
9.3.3 Environmental Health and Safety (EHS)   
9.4 Disinfection   
9.5 Treatment, Storage and Disposal   
9.5.1 General Storage and Disposal Methods for Different Types of Waste   
9.5.2 Chemical Treatment Standards   
9.5.3 Autoclave Standards   
9.5.4 Logging   
Appendix A Biological Safety Cabinets   
Appendix B Shipping of Biohazardous Materials   
Appendix C Transport of Biohazardous Material   
Appendix D Use of Human, Non-human Primate and other Mammalian Cells and Tissues   
Appendix E Guidelines for Work with Toxins of Biological Origin  
Appendix F NIH Oversight of Research Involving Recombinant DNA   
Appendix G Recombinant DNA Experiments Involving Plants   
Appendix H Autoclaves