Contents

Chapter 1 Guidance, Regulations and Roles of Key Personnel  1.1 Introduction 1.2 Guidance 1.3 Regulations 1.4 Roles of Key Personnel 1.4.1 Institutional Biosafety Committee (IBC) 1.4.2 Biological Safety Officer 1.4.3 Principal Investigator  Chapter 2 Biological Risk Assessment 2.1 Introduction 2.2 Hazardous Characteristics of Agents  2.2.1 Principal Hazardous Characteristics 2.2.2 Risk Groups  2.2.3 Resources  2.3 Risk Assessment for Human Cell Lines and Human-Derived Materials  2.4 Risk Assessment for Other Agents  Chapter 3- Principles of Biosafety  3.1 Introduction 3.2 Engineering Controls  3.3 Personal Protective Equipment  3.4 Facility Construction and Design  Chapter 4- Laboratory Biosafety Level Criteria  4.1 Introduction  4.2 Biosafety Level 1  4.3 Biosafety Level 2  4.4 Biosafety Level 3  4.5 Biosafety Level 4  4.6 Multi-User Research Labs  Chapter 5- Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities Chapter 6- Select Agents/ Toxins and Principles of Laboratory Biosecurity  Chapter 7- Medical Surveillance  Chapter 8 Emergencies and Reporting  8.1 Spills  8.1.1 General  8.1.2 Spills outside of a containment device  8.1.3 Spills inside of a Biological Safety Cabinet (BSC)  8.1.4 Spills in a centrifuge  8.1.5 Biological/Radioactive emergencies/spills 8.2 Exposure to Biohazardous Agents or Material  8.3 Security incidents  Chapter 9 Biohazardous Waste Management 9.1 Scope  9.2 Biohazardous Waste Definition  9.3 Responsibilities 9.3.1 Principal Investigator or Faculty in Charge of Lab 9.3.2 Biological Safety Officer (BSO)  9.3.3 Environmental Health and Safety (EHS)  9.4 Disinfection  9.5 Treatment, Storage and Disposal  9.5.1 General Storage and Disposal Methods for Different Types of Waste  9.5.2 Chemical Treatment Standards  9.5.3 Autoclave Standards  9.5.4 Logging  Appendix A Biological Safety Cabinets  Appendix B Shipping of Biohazardous Materials  Appendix C Transport of Biohazardous Material  Appendix D Use of Human, Non-human Primate and other Mammalian Cells and Tissues  Appendix E Guidelines for Work with Toxins of Biological Origin Appendix F NIH Oversight of Research Involving Recombinant DNA  Appendix G Recombinant DNA Experiments Involving Plants  Appendix H Autoclaves