Contents

Chapter 1 Guidance, Regulations and Roles of Key Personnel ................................................................................... 4
1.1 Introduction ......................................................................................................................................................... 4
1.2 Guidance .............................................................................................................................................................. 4
1.3 Regulations .......................................................................................................................................................... 4
1.4 Roles of Key Personnel ......................................................................................................................................... 4
1.4.1 Institutional Biosafety Committee (IBC) ........................................................................................................ 4
1.4.2 Biological Safety Officer ................................................................................................................................ 5
1.4.3 Principal Investigator .................................................................................................................................... 5
Chapter 2 Biological Risk Assessment ............................................................................................................................ 5
2.1 Introduction ......................................................................................................................................................... 5
2.2 Hazardous Characteristics of Agents ................................................................................................................... 6
2.2.1 Principal Hazardous Characteristics.............................................................................................................. 6
2.2.2 Risk Groups ................................................................................................................................................... 6
2.2.3 Resources ...................................................................................................................................................... 6
2.3 Risk Assessment for Human Cell Lines and Human-Derived Materials ................................................................ 7
2.4 Risk Assessment for Other Agents ....................................................................................................................... 7
Chapter 3- Principles of Biosafety .................................................................................................................................. 8
3.1 Introduction ......................................................................................................................................................... 8
3.2 Engineering Controls .................................................................................................................................... 8
3.3 Personal Protective Equipment .................................................................................................................... 8
3.4 Facility Construction and Design .................................................................................................................. 9
Chapter 4- Laboratory Biosafety Level Criteria .............................................................................................................. 9
4.1 Introduction ......................................................................................................................................................... 9
4.2 Biosafety Level 1 ................................................................................................................................................ 10
4.3 Biosafety Level 2 ................................................................................................................................................ 10
4.4 Biosafety Level 3 ................................................................................................................................................ 11
4.5 Biosafety Level 4 ................................................................................................................................................ 11
4.6 Multi-User Research Labs .................................................................................................................................. 11
Chapter 5- Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities ............................................. 12
Chapter 6- Select Agents/ Toxins and Principles of Laboratory Biosecurity ................................................................. 13
Arkansas State University Environmental Health
and Safety Biological Safety Manual | 3
Chapter 7- Medical Surveillance .................................................................................................................................. 13
Chapter 8 Emergencies and Reporting ........................................................................................................................ 14
8.1 Spills ................................................................................................................................................................... 14
8.1.1 General ....................................................................................................................................................... 14
8.1.2 Spills outside of a containment device ........................................................................................................ 14
8.1.3 Spills inside of a Biological Safety Cabinet (BSC) ......................................................................................... 15
8.1.4 Spills in a centrifuge .................................................................................................................................... 15
8.1.5 Biological/Radioactive emergencies/spills .................................................................................................. 16
8.2 Exposure to Biohazardous Agents or Material .................................................................................................. 16
8.3 Security incidents ............................................................................................................................................... 17
Chapter 9 Biohazardous Waste Management............................................................................................................. 17
9.1 Scope .................................................................................................................................................................. 17
9.2 Biohazardous Waste Definition ......................................................................................................................... 17
9.3 Responsibilities .................................................................................................................................................. 19
9.3.1 Principal Investigator or Faculty in Charge of Lab ...................................................................................... 19
9.3.2 Biological Safety Officer (BSO) .................................................................................................................... 19
9.3.3 Environmental Health and Safety (EHS) ...................................................................................................... 20
9.4 Disinfection ........................................................................................................................................................ 20
9.5 Treatment, Storage and Disposal ...................................................................................................................... 20
9.5.1 General Storage and Disposal Methods for Different Types of Waste ....................................................... 20
9.5.2 Chemical Treatment Standards .................................................................................................................. 21
9.5.3 Autoclave Standards ................................................................................................................................... 21
9.5.4 Logging ....................................................................................................................................................... 21
Appendix A Biological Safety Cabinets ......................................................................................................................... 22
Appendix B Shipping of Biohazardous Materials ......................................................................................................... 24
Appendix C Transport of Biohazardous Material ......................................................................................................... 24
Appendix D Use of Human, Non-human Primate and other Mammalian Cells and Tissues ...................................... 25
Appendix E Guidelines for Work with Toxins of Biological Origin ................................................................................ 26
Appendix F NIH Oversight of Research Involving Recombinant DNA ........................................................................... 26
Appendix G Recombinant DNA Experiments Involving Plants ..................................................................................... 27
Appendix H Autoclaves ................................................................................................................................................ 28