Contents

Chapter 1 Guidance, Regulations and Roles of Key Personnel ................................................................................... 4Personnel 
1.1 Introduction ......................................................................................................................................................... 4
1.2 Guidance .............................................................................................................................................................. 4
1.3 Regulations .......................................................................................................................................................... 4
1.4 Roles of Key Personnel ......................................................................................................................................... 4
1.4.1 Institutional Biosafety Committee (IBC) ........................................................................................................ 4
1.4.2 Biological Safety Officer ................................................................................................................................ 5
1.4.3 Principal Investigator .................................................................................................................................... 5Investigator 
Chapter 2 Biological Risk Assessment ............................................................................................................................ 5
2.1 Introduction ......................................................................................................................................................... 5
2.2 Hazardous Characteristics of Agents ................................................................................................................... 6Agents 
2.2.1 Principal Hazardous Characteristics.............................................................................................................. 6Characteristics
2.2.2 Risk Groups ................................................................................................................................................... 6Groups 
2.2.3 Resources ...................................................................................................................................................... 6Resources 
2.3 Risk Assessment for Human Cell Lines and Human-Derived Materials ................................................................ 7Materials 
2.4 Risk Assessment for Other Agents ....................................................................................................................... 7Agents 
Chapter 3- Principles of Biosafety .................................................................................................................................. 8Biosafety 
3.1 Introduction ......................................................................................................................................................... 8
3.2 Engineering Controls .................................................................................................................................... 8Controls 
3.3 Personal Protective Equipment .................................................................................................................... 8Equipment 
3.4 Facility Construction and Design .................................................................................................................. 9Design 
Chapter 4- Laboratory Biosafety Level Criteria .............................................................................................................. 9Criteria 
4.1 Introduction ......................................................................................................................................................... 9Introduction 
4.2 Biosafety Level 1 ................................................................................................................................................ 101 
4.3 Biosafety Level 2 ................................................................................................................................................ 102 
4.4 Biosafety Level 3 ................................................................................................................................................ 113 
4.5 Biosafety Level 4 ................................................................................................................................................ 114 
4.6 Multi-User Research Labs .................................................................................................................................. 11Labs 
Chapter 5- Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities ............................................. 12
Chapter 6- Select Agents/ Toxins and Principles of Laboratory Biosecurity ................................................................. 13
Arkansas State University Environmental Health
and Safety Biological Safety Manual | 3Biosecurity 
Chapter 7- Medical Surveillance .................................................................................................................................. 13Surveillance 
Chapter 8 Emergencies and Reporting ........................................................................................................................ 14Reporting 
8.1 Spills ................................................................................................................................................................... 14Spills 
8.1.1 General ....................................................................................................................................................... 14General 
8.1.2 Spills outside of a containment device ........................................................................................................ 14device 
8.1.3 Spills inside of a Biological Safety Cabinet (BSC) ......................................................................................... 15 
8.1.4 Spills in a centrifuge .................................................................................................................................... 15centrifuge 
8.1.5 Biological/Radioactive emergencies/spills .................................................................................................. 16
8.2 Exposure to Biohazardous Agents or Material .................................................................................................. 16Material 
8.3 Security incidents ............................................................................................................................................... 17incidents 
Chapter 9 Biohazardous Waste Management............................................................................................................. 17Management
9.1 Scope .................................................................................................................................................................. 17Scope 
9.2 Biohazardous Waste Definition ......................................................................................................................... 17Definition 
9.3 Responsibilities .................................................................................................................................................. 19
9.3.1 Principal Investigator or Faculty in Charge of Lab ...................................................................................... 19
9.3.2 Biological Safety Officer (BSO) .................................................................................................................... 19 
9.3.3 Environmental Health and Safety (EHS) ...................................................................................................... 20 
9.4 Disinfection ........................................................................................................................................................ 20Disinfection 
9.5 Treatment, Storage and Disposal ...................................................................................................................... 20Disposal 
9.5.1 General Storage and Disposal Methods for Different Types of Waste ....................................................... 20Waste 
9.5.2 Chemical Treatment Standards .................................................................................................................. 21Standards 
9.5.3 Autoclave Standards ................................................................................................................................... 21Standards 
9.5.4 Logging ....................................................................................................................................................... 21Logging 
Appendix A Biological Safety Cabinets ......................................................................................................................... 22Cabinets 
Appendix B Shipping of Biohazardous Materials ......................................................................................................... 24Materials 
Appendix C Transport of Biohazardous Material ......................................................................................................... 24Material 
Appendix D Use of Human, Non-human Primate and other Mammalian Cells and Tissues ...................................... 25Tissues 
Appendix E Guidelines for Work with Toxins of Biological Origin ................................................................................ 26
Appendix F NIH Oversight of Research Involving Recombinant DNA ........................................................................... 26DNA 
Appendix G Recombinant DNA Experiments Involving Plants ..................................................................................... 27Plants 
Appendix H Autoclaves ................................................................................................................................................ 28